Main sol. J987
Internal Solution-ID: S5017
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 0.991 | 18.528 | |
| K2HPO4 | 0.5 | g | 0.496 | 2.845 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.099 | 0.674 | |
| KCl | 0.8 | g | 0.793 | 10.635 | |
| Sodium acetate | 0.5 | g | 0.496 | 6.041 | |
| NaCl | 200.0 | g | 198.216 | 3391.79 | |
|
Yeast extract
(BD-Difco) |
1.0 | g | 0.991 | - | |
|
Tryptone
(BD-Difco) |
0.5 | g | 0.496 | 6.972 | |
| Trace element solution | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.496 | - | |
| Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 855.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
Glucose
(1 M) |
20.0 | ml | - | - | |
|
MgSO4 x 7 H2O
(10%) |
50.0 | ml | - | - | |
|
Tryptone
(10%) |
50.0 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere). | |||||
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Media with this solution
| J987 | CEJFB1T MEDIUM |