Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 3.0 | g | 3 | 22.703 | |
| KH2PO4 | 2.0 | g | 2 | 14.697 | |
| K2HPO4 | 7.0 | g | 7 | 40.189 | |
| NaCl | 2.0 | g | 2 | 34.223 | |
| MgSO4 x 7 H2O | 0.5 | g | 0.5 | 2.029 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Biotin | 10.0 | µg | 1.0e-5 | 4.1e-5 | |
| Thiamine-HCl x 2 H2O | 100.0 | µg | 1.0e-4 | 2.7e-4 | |
| MnSO4 x H2O | 6.2 | mg | 0.006 | 0.037 | |
| FeSO4 x 7 H2O | 10.0 | mg | 0.01 | 0.036 | |
| Methanol | 10.0 | ml | - | - | |
| Agar , if necessary | 15.0 | g | 15 | 44.599 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.2 prior to autoclaving. | |||||
Media with this solution
| 242 | BMM - MEDIUM |