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Basic FWM solution

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Internal Solution-ID: S5041

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.0 g 0.983 16.826
KCl 0.5 g 0.492 6.595
MgCl2 x 6 H2O 0.4 g 0.393 1.935
KH2PO4 0.2 g 0.197 1.445
CaCl2 x 2 H2O 0.1 g 0.098 0.669
Distilled water 1000.0 ml - -
1 Autoclave Basic FWM solution and aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order:
Modified trace element mixture 1.0 ml - -
Vitamin solution
(*) 		1.0 ml - -
FeNa-EDTA
(pH 7.0, *, 7.5 mM) 		1.0 ml - -
NaHCO3
(*, 1 M) 		2.0 ml - -
HEPES solution 10.0 ml - -
NH4Cl
(1 M) 		1.0 ml - -
Sodium pyruvate
(*, 1 M) 		1.0 ml - -
2 Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking.
J1004 FRESHWATER MEDIUM FOR SOIL AOA