Main sol. J1020
Internal Solution-ID: S5066
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2SO4 | 2.000 | g | 1.843 | 12.978 | |
NaCl | 8.800 | g | 8.111 | 138.785 | |
MgCl2 x 6 H2O | 4.600 | g | 4.24 | 20.854 | |
KCl | 0.340 | g | 0.313 | 4.203 | |
NH4Cl | 0.250 | g | 0.23 | 4.308 | |
KH2PO4 | 0.140 | g | 0.129 | 0.948 | |
CaCl2 x 2 H2O | 0.140 | g | 0.129 | 0.878 | |
NiCl2 x 6 H2O | 9.300 | mg | 0.009 | 0.066 | |
Fe(NH4)2(SO4)2 x 6 H2O | 2.700 | mg | 0.002 | - | |
Na2SeO3 x 5 H2O | 0.260 | mg | 2.4e-4 | 9.1e-4 | |
Na2WO4 x 2 H2O | 0.084 | mg | 7.7e-5 | 2.3e-4 | |
Trace element solution | 10.000 | ml | - | - | |
Trace vitamins | 10.000 | ml | - | - | |
Sodium benzoate | 0.360 | g | 0.332 | 2.303 | |
Resazurin | 1.000 | mg | 9.2e-4 | 0.004 | |
Distilled water | 1000.000 | ml | - | - | |
1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 10 ml medium in Balch-type tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions: | |||||
NaHCO3
(filter-sterilized, 8%) |
60.000 | ml | - | - | |
Na2S x 9 H2O
(autoclaved, 5%) |
5.000 | ml | - | - |
J1020 | DESULFOCARBO INDIANENSIS MEDIUM |