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Main sol. J1024

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Internal Solution-ID: S5074

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		1.00 g 0.999 -
Tryptone
(BD-Difco) 		1.00 g 0.999 14.055
Nitrilotriacetic acid 0.10 g 0.1 0.523
CaSO4 x 2 H2O 0.04 g 0.04 0.232
MgCl2 x 6 H2O 0.20 g 0.2 0.983
Iron(III) citrate
(0.01 M) 		0.50 ml - -
Nitsch's trace elements 0.50 ml - -
Distilled water 900.00 ml - -
1 Adjust pH to 7.2 with NaOH. For preparation of solid medium, add 28.0 g/L agar. After autoclaving, add the following solution to the medium:
Phosphate buffer 100.00 ml - -
J1024 THERMUS 162 MEDIUM