Main sol. J1026
Internal Solution-ID: S5077
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 0.999 | 18.676 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.202 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.721 | |
| NaCl | 25.0 | g | 24.975 | 427.362 | |
| KCl | 0.1 | g | 0.1 | 1.34 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Yeast extract | 1.0 | g | 0.999 | - | |
| Trace element solution | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 925.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]: | |||||
|
MgCl2 x 6 H2O
(15%) |
20.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
Glucose
(1 M) |
20.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
| 2 Readjust pH to 7.2, if necessary. | |||||
Media with this solution
| J1026 | IRD MARINIFILUM MEDIUM |