Main sol. J1037
Internal Solution-ID: S5093
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Maltose monohydrate | 0.5 | g | 0.494 | 1.37 | |
| Sodium pyruvate | 0.4 | g | 0.395 | 3.588 | |
|
Casamino acids
(BD-Difco) |
0.3 | g | 0.296 | - | |
|
Yeast extract
(BD-Difco) |
0.3 | g | 0.296 | - | |
| Solution A | 5.0 | ml | - | - | |
| Solution B | 8.0 | ml | - | - | |
| Agar | 12.0 | g | 11.846 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Before autoclaving, adjust pH to 7.6 - 7.8 for agar plate and to 7.4 - 7.6 for liquid medium. Autoclave at 115C for 20 min. Separately sterilize Solution B by filtration and add to the medium aseptically. | |||||
Media with this solution
| J1037 | MPYCY AGAR MEDIUM |