Main sol. 249
Internal Solution-ID: S513
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD BACTO) |
5.00 | g | 5 | 70.343 | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| KH2PO4 | 1.36 | g | 1.36 | 9.994 | |
| K2HPO4 | 6.95 | g | 6.95 | 39.902 | |
| Na-orotate | 2.50 | g | 2.5 | 14.039 | |
| Riboflavin | 15.00 | mg | 0.015 | 0.04 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Na-thioglycolate | 0.50 | g | 0.5 | 4.382 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients and adjust pH to 7.5. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Media with this solution
| 249 | FAECALICATENA OROTICA MEDIUM |