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Main sol. J1065

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Internal Solution-ID: S5130

Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.291 5.435
Na2SO4 1.42 g 1.376 9.687
CaCl2 x 2 H2O 0.11 g 0.107 0.725
MgCl2 x 6 H2O 0.10 g 0.097 0.477
NaCl 0.30 g 0.291 4.974
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Yeast extract
(BD-Difco) 		0.20 g 0.194 -
Trace vitamins 10.00 ml - -
Resazurin 0.50 mg 4.8e-4 0.002
Distilled water 1000.00 ml - -
1 Mix components thoroughly, bubble with a N2-CO2 (80:20, v/v) gas mixture, seal with a butyl rubber stopper and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (separately autoclaved under a N2 atmosphere):
K2HPO4
(1 M) 		5.00 ml - -
glycerin
(1 M) 		5.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
J1065 ACIDIFIED MPOB MEDIUM