Main sol. J1066
Internal Solution-ID: S5131
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MES | 3.90 | g | 3.816 | 19.545 | |
| NaCl | 25.00 | g | 24.462 | 418.58 | |
| MgSO4 x 7 H2O | 2.70 | g | 2.642 | 10.719 | |
| MgCl2 x 6 H2O | 4.30 | g | 4.207 | 20.695 | |
| NH4Cl | 0.25 | g | 0.245 | 4.573 | |
| KCl | 0.34 | g | 0.333 | 4.463 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.137 | 0.932 | |
| K2HPO4 | 0.14 | g | 0.137 | 0.786 | |
| Yeast extract | 0.20 | g | 0.196 | - | |
| Trace elements | 1.00 | ml | - | - | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(in 0.037% HCl, 0.2%) |
1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 5.5. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter sterilized): | |||||
|
Trace vitamins (see Medium No. 197)
(*) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
Media with this solution
| J1066 | M25Y MEDIUM |