Main sol. J1068
Internal Solution-ID: S5133
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Artificial saltwater | 1000.0 | ml | - | - | |
| Wolfe's mineral solution | 10.0 | ml | - | - | |
| 1 Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized): | |||||
| Trace vitamins | 10.0 | ml | - | - | |
|
NaHCO3
(8%) |
12.5 | ml | - | - | |
|
FeCl2
(freshly prepared, 0.2 M) |
10.0 | ml | - | - | |
| 2 Replace the gas phase with a N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture. | |||||
Media with this solution
| J1068 | SALTWATER IRON-OXIDIZING BACTERIA MEDIUM-II |