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Main sol. J1069

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Internal Solution-ID: S5134

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial saltwater 1000.00 ml - -
MES 1.95 g 1.929 9.879
Wolfe's mineral solution 1.00 ml - -
Trace vitamins 10.00 ml - -
Tryptone
(BD-Difco) 		2.00 g 1.978 27.831
1 Mix components and adjust pH to 6.2 to 6.5. Distribute the medium into culture vessels, replace the gas phase with N2-CO2-O2 (90:10:2, v/v/v), seal with butyl rubber stoppers and autoclave.
J1069 MICROAEROPHILIC LUTIBACTER MEDIUM