Main sol. J1078
Internal Solution-ID: S5143
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.0 | g | 0.993 | 16.993 | |
| NH4Cl | 1.0 | g | 0.993 | 18.565 | |
| KH2PO4 | 0.1 | g | 0.099 | 0.73 | |
| K2HPO4 | 0.1 | g | 0.099 | 0.57 | |
| KCl | 0.1 | g | 0.099 | 1.332 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.099 | 0.488 | |
| Tris base | 3.0 | g | 2.979 | 24.593 | |
| Na2S2O3 x 5 H2O | 1.5 | g | 1.49 | 6.002 | |
| Trace element solution | 1.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 910.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 9.0 with NaOH and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Add the following solutions from sterile anaerobic stocks (autoclaved and stored under a N2 atmosphere): | |||||
|
Yeast extract
(w/v, 10%) |
50.0 | ml | - | - | |
|
Crotonic acid
(pH 7.0, 0.5 M) |
20.0 | ml | - | - | |
|
Na2CO3
(8%) |
20.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
6.0 | ml | - | - | |
| 2 Check final pH be around 8.5. | |||||
Media with this solution
| J1078 | ALKALIPHILUS 3B MEDIUM |