Main sol. J1079
Internal Solution-ID: S5144
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 240.0 | g | 8571.43 | 146671 | |
| K2HPO4 | 2.5 | g | 89.286 | 512.618 | |
| NH4Cl | 0.5 | g | 17.857 | 333.841 | |
| HEPES | 7.0 | g | 250 | 1049.05 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Autoclave and, after cooling, add per liter the following solutions (autoclaved or *filter-sterilized separately): | |||||
|
MgCl2
(1 M) |
1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
|
NaHCO3
(*, 1 M) |
10.0 | ml | - | - | |
| 2 To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 1.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Sodium pyruvate
(*, 2 M) |
10.0 | ml | - | - | |
|
Yeast extract
(10%) |
0.2 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
5.0 | ml | - | - | |
| 3 Basal mineral NaCl medium: | |||||
Media with this solution
| J1079 | HALANAEROARCHAEUM MEDIUM |