Main sol. J1093
Internal Solution-ID: S5164
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
| NH4Cl | 0.25 | g | 0.123 | 2.307 | |
| KNO3 | 0.25 | g | 0.123 | 1.221 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized): | |||||
|
NaHCO3
(8%) |
10.00 | ml | - | - | |
|
Na2S2O3 x 5 H2O
(10%) |
15.00 | ml | - | - | |
| Trace vitamins | 1.00 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2 (80:20, v/v) gas mixture, and pressurize to 200 kPa. Adjust pH to 6.0, if necessary. | |||||
Media with this solution
| J1093 | MJ BASAL MEDIUM WITH THIOSULFATE/2 |