Main sol. 253
Internal Solution-ID: S517
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.495 | 3.634 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.049 | 0.336 | |
| NH4Cl | 0.80 | g | 0.791 | 14.793 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.099 | 0.487 | |
| NaCl | 180.00 | g | 178.042 | 3046.57 | |
| Na2SO4 | 20.00 | g | 19.782 | 139.273 | |
| Na2CO3 | 6.00 | g | 5.935 | 55.994 | |
| Trace element solution (A) | 1.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.495 | - | |
| Sodium succinate | 1.00 | g | 0.989 | 6.104 | |
| Distilled water | 800.00 | ml | - | - | |
| 1 Adjust pH to 8.5. | |||||
| 2 Bubble with nitrogen gas and fill in tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. After sterilization add 1 ml/l filter-sterilized vitamin solution (B), 200 ml of filter-sterilized solution Bicarbonate solution (C) and 10 ml/l sterilised Na2S x 9H2O solution (D). pH 8.5 | |||||
| Vitamin solution (B) | 1.00 | ml | - | - | |
| Bicarbonate solution (C) | 200.00 | ml | - | - | |
| Na2S x 9H2O solution (D) | 10.00 | ml | - | - | |
Media with this solution
| 253 | MEDIUM FOR ECTOTHIORHODOSPIRA |