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Main sol. J1098

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Internal Solution-ID: S5173

Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 3.620 g 3.62 61.944
K2HPO4 0.260 g 0.26 1.493
NH4Cl 0.075 g 0.075 1.402
Na2SO4 0.050 g 0.05 0.352
NaNO3 0.850 g 0.85 10.001
L-Sodium lactate 2.240 g 2.24 19.989
Yeast extract 0.200 g 0.2 -
Tryptone 0.500 g 0.5 7.034
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
Trace vitamins 10.000 ml - -
Distilled water 935.500 ml - -
1 Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, add the following solution (filter-sterilized):
NaHCO3
(8%) 		52.500 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels under a N2 atmosphere and add H2 at 5% volume of the gas phase.
J1098 BSM-RC MEDIUM