Main sol. J1112
Internal Solution-ID: S5201
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 2.5 | g | 2.444 | 41.817 | |
| KCl | 0.3 | g | 0.293 | 3.934 | |
| K2HPO4 | 0.3 | g | 0.293 | 1.684 | |
| MgSO4 x 7 H2O | 0.1 | g | 0.098 | 0.397 | |
| NH4Cl | 1.0 | g | 0.978 | 18.275 | |
| CaSO4 x 7 H2O | 12.5 | mg | 0.012 | 0.09 | |
| Yeast extract | 0.1 | g | 0.098 | - | |
| Trace vitamins | 2.0 | ml | - | - | |
| Trace minerals solution | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.4 and autoclave. After cooling, add the following solution (autoclaved): | |||||
|
Glucose
(1 M) |
20.0 | ml | - | - | |
Media with this solution
| J1112 | MINERAL MEDIUM WITH GLUCOSE |