Main sol. J1113
Internal Solution-ID: S5202
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 2.5 | g | 2.425 | 41.493 | |
KCl | 0.3 | g | 0.291 | 3.903 | |
K2HPO4 | 0.3 | g | 0.291 | 1.671 | |
MgSO4 x 7 H2O | 0.1 | g | 0.097 | 0.394 | |
NH4Cl | 1.0 | g | 0.97 | 18.133 | |
CaSO4 x 7 H2O | 12.5 | mg | 0.012 | 0.089 | |
Yeast extract | 1.0 | g | 0.97 | - | |
Fatty acid mixture | 10.0 | ml | - | - | |
Trace vitamins | 2.0 | ml | - | - | |
Trace minerals solution | 1.0 | ml | - | - | |
Resazurin | 0.5 | mg | 4.8e-4 | 0.002 | |
Distilled water | 1000.0 | ml | - | - | |
1 Mix components, adjust pH to 7.0 and autoclave under a N2 atmosphere in a bottle capped with a butyl rubber stopper. After cooling, add the following solution (autoclaved and stored under a N2 gas atmosphere): | |||||
Sodium Thiosulfate
(1.0 M) |
10.0 | ml | - | - | |
Na2S x 9 H2O
(pH 7.0, 5%) |
8.0 | ml | - | - |
J1113 | ANAEROBIC MINERAL MEDIUM WITH YEAST EXTRACT |