Main sol. 255
Internal Solution-ID: S521
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.499 | 3.667 | |
| K2HPO4 x 3 H2O | 1.00 | g | 0.998 | 5.73 | |
| Urea | 2.00 | g | 1.996 | 33.236 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.499 | 2.454 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.339 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
1.25 | ml | 0.001 | 0.004 | |
| Morpholinopropane sulfonic acid | 10.00 | g | 9.98 | 47.692 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Yeast extract | 6.00 | g | 5.988 | - | |
| L-Cysteine HCl x H2O | 1.00 | g | 0.998 | 5.682 | |
| Cellobiose | 5.00 | g | 4.99 | 14.578 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine and cellobiose and adjust pH to 7.2. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and readjust pH to 7.2, if necessary. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. | |||||
Media with this solution
| 255 | CLOSTRIDIUM (GS) MEDIUM |