Basic mineral salt medium 1
Internal Solution-ID: S5243
This solution is used in 2 media and was first defined in medium J1139.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 240.0 | g | 120000 | 2053390 | |
| K2HPO4 | 2.5 | g | 1250 | 7176.65 | |
| (NH4)2SO4 | 0.5 | g | 250 | 1891.93 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 - 6.9 by 1 M K2HPO4 and autoclave. After cooling, add the following solutions (autoclaved): | |||||
|
MgCl2
(1 M) |
1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |