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Casein solution 5%(w/v)

Internal Solution-ID: S5245

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein 5 g 50 24.248
Distilled water 100 ml - -
1 Suspend casein in distilled water and keep at 50C. Titrate pH to 8.2 - 8.5 with 1 M NaOH solution, until pH is stabilized. Autoclave the solution at 120C for 40 min in a closed bottle.
J1139 NATRONOSPIRA PROTEINIVORA MEDIUM