Main sol. J1142
Internal Solution-ID: S5248
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.080 | g | 0.077 | 0.523 | |
| MgCl2 x 6 H2O | 0.200 | g | 0.192 | 0.946 | |
| NaCl | 10.000 | g | 9.615 | 164.534 | |
| KCl | 1.000 | g | 0.962 | 12.898 | |
| NH4Cl | 1.000 | g | 0.962 | 17.976 | |
| PIPES | 1.000 | g | 0.962 | 3.18 | |
| Yeast extract | 0.500 | g | 0.481 | - | |
| FeCl2 solution | 1.000 | ml | - | - | |
| Trace element solution | 1.000 | ml | - | - | |
| Trace vitamins | 1.000 | ml | - | - | |
| Selenite-tungstate solution | 1.000 | ml | - | - | |
| Resazurin | 1.000 | mg | 9.6e-4 | 0.004 | |
| Phosphate buffer | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (80:20, v/v) gas mixture. When the medium is cooled to around 50C, add the following components: | |||||
| NaHCO3 | 2.000 | g | 1.923 | 22.892 | |
| L-Cysteine HCl x H2O | 0.031 | g | 0.03 | - | |
| 2 Continue flushing with the gas mixture until the chemicals are dissolved completely. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclave and stored under a N2 atmosphere): | |||||
|
Glucose
(1.0 M) |
10.000 | ml | - | - | |
|
Ferric citrate
(pH 7.0, 0.25 M) |
20.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
0.500 | ml | - | - | |
Media with this solution
| J1142 | TEPIDIBACILLUS DECATURENSIS MEDIUM |