Main sol. J1181
Internal Solution-ID: S5292
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MDS salt water | 833.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
|
Yeast extract
(BD-Difco) |
0.1 | g | 0.114 | - | |
| 1 Add components and bring volume to 958 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar: see Medium No. 574). Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium. | |||||
|
NH4Cl
(1 M) |
5.0 | ml | - | - | |
| Potassium phosphate buffer | 2.0 | ml | - | - | |
|
Trace vitamins
(*) |
5.0 | ml | - | - | |
|
Cellobiose
(0.2 M) |
30.0 | ml | - | - | |
| 2 Readjust pH to 7.0 with a sterilized 10% Na2CO3 solution, if necessary. | |||||
Media with this solution
| J1181 | MODIFIED CELLULOLYTIC HALOARCHAEA MEDIUM |