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Main sol. J1183

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Internal Solution-ID: S5295

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 0.499 3.667
NH4Cl 0.5 g 0.499 9.329
MgCl2 x 6 H2O 0.3 g 0.299 1.473
KCl 0.5 g 0.499 6.694
NaCl 0.5 g 0.499 8.539
Na2S2O3 x 5 H2O 0.1 g 0.1 0.402
Yeast extract
(BD-Difco) 		1.0 g 0.998 -
HEPES 3.0 g 2.994 12.564
Trace vitamins solution 1.0 ml - -
Trace elements solution 1.0 ml - -
Distilled water 1000.0 ml - -
1 After autoclaving, add 1 ml of 5% Na2S x 9H2O solution (autoclaved), 1 ml of 5% CaCl2 x 2H2O solution (autoclaved) and 10 ml of 3% NaHCO3 solution (filter-sterilized). Adjust pH to 7.5.
J1183 CHLOROFLEXUS MEDIUM