Main sol. J1227
Internal Solution-ID: S5357
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Brock's salt base solution | 1000.0000 | ml | - | - | |
| Na2S2O3 x 5 H2O | 2.0000 | g | 0.995 | 4.009 | |
| NaOH | 0.1600 | g | 0.08 | 1.99 | |
| Resazurin | 1.0000 | mg | 5.0e-4 | 0.002 | |
| Trace mineral solution | 10.0000 | ml | - | - | |
| Distilled water | 1000.0000 | ml | - | - | |
| 1 Heat the medium to boiling while bubbling with a N2-CO2 (4:1, v/v) gas mixture. After boiling, cool the medium in a water bath. Continue bubbling with the same gas mixture. While the medium is still warm, add the following: | |||||
|
Yeast extract
(Oxoid) |
1.0000 | g | 0.498 | - | |
| Tryptone | 1.0000 | g | 0.498 | 6.999 | |
|
Casamino acids
(BD-Difco) |
0.5000 | g | 0.249 | - | |
| L-Cysteine HCl x H2O | 0.0625 | g | 0.031 | - | |
| 2 After the medium is cooled to room temperature, adjust pH to 6.0 - 6.1. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
Media with this solution
| J1227 | ZESTOSPHAERA MEDIUM |