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Main sol. J1244

Internal Solution-ID: S5380

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Marine broth 2216
(BD-Difco)
37.4 g 37.03 -
Distilled water 1000.0 ml - -
1 Mix components and adjust pH to 8.0. Autoclave. After cooling, add the following solution (autoclaved):
Sodium pyruvate
(1.0 M)
10.0 ml - -
2 Comments: Remove precipitate yielded after autoclaving by filtration, if necessary.
J1244 MARINE BROTH 2216 WITH PYRUVATE