Main sol. J1245
Internal Solution-ID: S5381
Equipment needed: Anaerobic workbench
Hungate tubes
Filter
Autoclave
| 1 Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand overnight. Aseptically and anaerobically add the following solutions (filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.00 | g | 1.008 | 17.25 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.202 | 0.992 | |
| NaH2PO4 x 2 H2O | 0.91 | g | 0.917 | 7.646 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.101 | 0.686 | |
| NH4Cl | 1.00 | g | 1.008 | 18.846 | |
|
Yeast extract
(BD-Difco) |
0.20 | g | 0.202 | - | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Distilled water | 900.00 | ml | - | - | |
|
NaHCO3
(8.0%) |
30.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
|
Lactose
(0.25 M) |
50.00 | ml | - | - | |
| 2 Readjust pH to 7.2-7.6, if necessary. | |||||
Media with this solution
| J1245 | TRICHOCOCCUS SKBG MEDIUM |