Main sol. J1278
Internal Solution-ID: S5422
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Wolfe's mineral solution | 1000.00 | ml | - | - | |
| Trace minerals | 10.00 | ml | - | - | |
| MES | 1.95 | g | 1.893 | 9.697 | |
| NaHCO3 | 0.83 | g | 0.806 | 9.592 | |
| Sodium acetate | 1.36 | g | 1.32 | 16.096 | |
| Sodium pyruvate | 1.10 | g | 1.068 | 9.705 | |
| Sodium fumarate | 3.20 | g | 3.107 | 19.413 | |
| 1 Mix components thoroughly and adjust pH 6.2. Autoclave under a N2 atmosphere. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and stored under a N2 atmosphere): | |||||
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Media with this solution
| J1278 | MIZ13 MEDIUM |