Main sol. J1315
Internal Solution-ID: S5475
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 0.450 | g | 0.437 | 7.476 | |
| NaHCO3 | 0.200 | g | 0.194 | 2.311 | |
| MgSO4 x 7 H2O | 0.090 | g | 0.087 | 0.355 | |
| KH2PO4 | 0.225 | g | 0.218 | 1.605 | |
| K2HPO4 | 0.225 | g | 0.218 | 1.254 | |
| (NH4)2SO4 | 0.225 | g | 0.218 | 1.653 | |
| CaCl2 x 2 H2O | 0.060 | g | 0.058 | 0.396 | |
| (NH4)2Ni(SO4)2 x 6 H2O | 2.000 | mg | 0.002 | 0.005 | |
| FeSO4 x 7 H2O | 2.000 | mg | 0.002 | 0.007 | |
| Wolfe's mineral elixir | 0.100 | ml | - | - | |
| Resazurin | 1.000 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly, except NaHCO3, and adjust pH to 7.0. Bring to a boil for a seconds, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter the following solutions (autoclaved or filter-sterilized, and stored under N2): | |||||
|
Trace vitamins
(*) |
10.000 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
10.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.000 | ml | - | - | |
| 2 Pressurize inoculated bottles to 100 KPa H2-CO2 (80:20, v/v). | |||||
Media with this solution
| J1315 | METHANOGEN MS-I MEDIUM |