Main sol. J1319
Internal Solution-ID: S5480
Equipment needed: Hungate tubes
Anaerobic workbench
Filter
Autoclave
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.98 | 18.329 | |
| KH2PO4 | 0.33 | g | 0.324 | 2.377 | |
| K2HPO4 | 0.45 | g | 0.441 | 2.533 | |
| NaCl | 10.00 | g | 9.804 | 167.76 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.098 | 0.398 | |
| Trace minerals | 10.00 | ml | - | - | |
| Yeast extract | 2.00 | g | 1.961 | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 910.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
50.00 | ml | - | - | |
|
Fructose
(1.0 M) |
20.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
| 2 Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions (autoclaved and stored under N2): | |||||
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Media with this solution
| J1319 | MODIFIED ACETOBACTER MEDIUM |