Main sol. 286
Internal Solution-ID: S553
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.30 | g | 1.3 | 9.553 | |
| K2HPO4 | 7.00 | g | 7 | 40.189 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
| FeCl3 x 6 H2O | 0.01 | g | 0.01 | 0.037 | |
| CaCl2 x 2 H2O | 0.01 | g | 0.01 | 0.068 | |
|
Na2MoO4 x 2 H2O
(0.1% w/v) |
1.00 | ml | 1.0e-3 | 0.004 | |
| Yeast extract | 3.00 | g | 3 | - | |
| gamma-Aminobutyric acid | 5.00 | g | 5 | 48.487 | |
| Agar | 1.50 | g | 1.5 | 4.46 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2S x 9 H2O | 0.30 | g | 0.3 | 1.249 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfide), adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. | |||||
Media with this solution
| 286 | CLOSTRIDIUM AMINOBUTYRICUM MEDIUM |