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Main sol. 26

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Internal Solution-ID: S5551

Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.20 g 0.2 -
Disodium succinate 1.00 g 1 6.171
Ammonium acetate 0.50 g 0.5 6.487
Fe(III) citrate
(0.1% in H2O) 		5.00 ml 0.005 0.02
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NaCl 0.40 g 0.4 6.845
NH4Cl 0.40 g 0.4 7.478
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Vitamin B12
(10 mg in 100 ml H2O) 		0.40 ml - -
Trace element solution SL-6 1.00 ml - -
L-Cysteine HCl 0.30 g 0.3 1.903
Resazurin
(0,1%) 		0.50 ml 0.005 0.022
Distilled water 1000.00 ml - -
1 Adjust pH to 5.7.
2 Boil the medium for a few minute.
3 Sparge medium with 100% N2 gas mixture to make it anoxic.
4 Fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas.
5 Autoclave at 121 °C for 20 min.
6 Sterile syringes are used to inoculate and remove samples.
7 Incubate in the light using a tungsten lamp.
26 ACID RHODOSPIRILLACEAE MEDIUM