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Soil extract

Internal Solution-ID: S5555

Compound Amount Unit Conc. [g/L] Conc. [mM]
air-dried garden soil 80.0 g 400 -
Na2CO3 0.2 g 1 9.435
Distilled water 200.0 ml - -
1 Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2.
98 RHIZOBIUM MEDIUM