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Main sol. 88b

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Internal Solution-ID: S5562

Equipment needed: Autoclave
1 Prepare medium , then add (per litre):
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.3 9.838
KH2PO4 0.28 g 0.28 2.058
MgSO4 x 7 H2O 0.25 g 0.25 1.014
CaCl2 x 2 H2O 0.07 g 0.07 0.476
FeCl3 x 6 H2O 0.02 g 0.02 0.074
Allen's trace element solution 10.00 ml - -
Distilled water 1000.00 ml - -
Sulfur , powdered
(powdered) 		1.00 g 1 31.182
Yeast extract 0.20 g 0.2 -
Starch , soluble
(soluble) 		2.00 g 2 -
Resazurin 0.40 mg 4.0e-4 0.002
Na2S x 9 H2O 0.50 g 0.5 2.082
2 Adjust the pH to 6.0 with H2SO4 (25% v/v). Prepare the medium under 100% N2 gas phase and distribute anaerobically into rubber stoppered tubes or bottles (presterilized by autoclaving). The medium is sterilized by heating 2 hours at 85 °C on each of two following days.
88b V24N MEDIUM