MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 109

Download this solution as

CSV JSON PDF

Internal Solution-ID: S5572

Equipment needed: Tungsten lamp Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.30 g 0.3 -
Disodium succinate 1.00 g 1 6.171
Ammonium acetate 0.50 g 0.5 6.487
Fe(III) citrate
(0.1% in H2O) 		5.00 ml 0.005 0.02
KH2PO4 0.50 g 0.5 3.674
MgSO4 x 7 H2O 0.40 g 0.4 1.623
NaCl 0.40 g 0.4 6.845
NH4Cl 0.40 g 0.4 7.478
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Vitamin B12
(10 mg in 100 ml H2O) 		0.40 ml - -
Trace element solution SL-6 1.00 ml - -
L-Cysteine HCl 0.30 g 0.3 1.903
Resazurin
(0,1%) 		0.50 ml 0.005 0.022
Distilled water 1000.00 ml - -
1 To medium add 0.1% sodium malate and 0.05% sodium thiosulfate (). Adjust pH to 6.8 and sterilize at 121°C for 15 min. The liquid medium is sterilized in rubber stoppered or screw-capped bottles under anaerobic conditions (Ref. 3365). After sterilization neutralized sterile sodium sulfide solution is added to a final concentration of 0.03% (see medium 27).
Sodium malate 0.10 % 1 5.616
Sodium Thiosulfate 0.05 % 0.5 3.162
2 Incubate in the light using a tungsten lamp.
3 Adjust pH to 6.8.
109 RHODOPSEUDOMONAS SULFOVIRIDIS MEDIUM