Main sol. 160
Internal Solution-ID: S5576
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Casitone | 3.00 | g | 3 | - | |
CaCl2 x 2 H2O | 1.36 | g | 1.36 | 9.251 | |
Yeast extract | 1.00 | g | 1 | - | |
Agar , alternative | 15.00 | g | 15 | 44.599 | |
Cellobiose
, alternative
(filter-sterilized) |
0.50 | % | 5 | 14.607 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 7.2. | |||||
2 To medium add 0.5% cellobiose (filter-sterilized) or distribute medium (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium. |
160 | CYTOPHAGA HUTCHINSONII MEDIUM |