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Main sol. 160

Internal Solution-ID: S5576

Compound Amount Unit Conc. [g/L] Conc. [mM]
Casitone 3.00 g 3 -
CaCl2 x 2 H2O 1.36 g 1.36 9.251
Yeast extract 1.00 g 1 -
Agar , alternative 15.00 g 15 44.599
Cellobiose , alternative
(filter-sterilized)
0.50 % 5 14.607
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2.
2 To medium add 0.5% cellobiose (filter-sterilized) or distribute medium (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium.
160 CYTOPHAGA HUTCHINSONII MEDIUM