Main sol. 36
Internal Solution-ID: S56
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 0.10 | g | 0.1 | 0.757 | |
K2HPO4 | 4.00 | g | 4 | 22.965 | |
KH2PO4 | 4.00 | g | 4 | 29.393 | |
MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
CaCl2 | 0.10 | g | 0.1 | 0.901 | |
FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.074 | |
MnSO4 x H2O | 0.02 | g | 0.02 | 0.118 | |
Agar
, for solid medium
optional |
12.00 | g | 12 | 35.679 | |
Na2S2O3 x 5 H2O | 10.00 | g | 10 | 40.292 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable culivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. |
36 | THIOBACILLUS MEDIUM |