Main sol. 764
Internal Solution-ID: S5653
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| MgCl2 x 6 H2O | 2.0 | g | 2 | 9.838 | |
| CaCl2 x 2 H2O | 0.2 | g | 0.2 | 1.36 | |
| KCl | 4.0 | g | 4 | 53.655 | |
| Na-acetate | 1.0 | g | 1 | 12.19 | |
| Glucose | 10.0 | g | 10 | 55.506 | |
| Trypticase | 0.5 | g | 0.5 | - | |
| NaCl | 200.0 | g | 200 | 3422.31 | |
| Modified Wolin's mineral solution II | 1.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the medium and boil, cool under N2:CO2 (80:20 v/v). Dispense medium into Hungate tubes or serum bottles under N2:CO2 (80:20 v/v), autoclave. To the sterile medium add from sterile stock solutions 2% Na2S x 9 H2O (0.1ml/10ml), 10% NaHCO3 (0.5ml/10ml), and 1M glucose (0.25ml/10ml). Final pH 7.0. | |||||
Media with this solution
| 764 | HALOANAEROBIUM LACUSROSEUS MEDIUM |