Main sol. 311a
Internal Solution-ID: S577
Equipment needed: Autoclave
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.50 | g | 0.498 | 9.301 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.498 | 2.019 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.249 | 1.692 | |
| NaCl | 2.25 | g | 2.239 | 38.31 | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
2.00 | ml | 0.002 | 0.007 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 2.00 | g | 1.99 | - | |
| Casitone | 2.00 | g | 1.99 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| K2HPO4 | 0.35 | g | 0.348 | 1.999 | |
| KH2PO4 | 0.23 | g | 0.229 | 1.682 | |
| Na2CO3 | 1.00 | g | 0.995 | 9.388 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| DL-Dithiothreitol | 0.15 | g | 0.149 | 0.967 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except phosphates, carbonate, vitamins, and dithiothreitol) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under the same gas atmosphere in anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add phosphates to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and dithiothreitol should be prepared under N2 gas and sterilized by filtration. Adjust the pH of the completed medium to pH 7.0. | |||||
Media with this solution
| 311a | SPOROMUSA MEDIUM (H2/CO2) |