Main sol. 38
Internal Solution-ID: S58
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 20.00 | g | 20 | 281.373 | |
| Yeast extract | 10.00 | g | 10 | - | |
| K2HPO4 | 1.04 | g | 1.04 | 5.971 | |
| KH2PO4 | 0.68 | g | 0.68 | 4.997 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2S x 9 H2O | 0.15 | g | 0.15 | 0.625 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. | |||||
Media with this solution
| 38 | ACETOANAEROBIUM MEDIUM |