Main sol. 312
Internal Solution-ID: S580
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sucrose | 1.00 | g | 1 | 2.921 | |
| Casitone | 0.75 | g | 0.75 | - | |
| Yeast extract | 0.25 | g | 0.25 | - | |
|
Trypticase soy broth
(without dextrose, BBL) |
0.17 | g | 0.17 | - | |
| Thiamine-HCl x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.1. | |||||
| 2 Sterilize vitamins separately by filtration. | |||||
Media with this solution
| 312 | SCY - MEDIUM |