Main sol. 314
Internal Solution-ID: S581
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 21.00 | g | 20.937 | 358.268 | |
| MgCl2 x 6 H2O | 3.10 | g | 3.091 | 15.203 | |
| KCl | 0.30 | g | 0.299 | 4.012 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.017 | |
| NH4Cl | 0.27 | g | 0.269 | 5.033 | |
| KH2PO4 | 0.20 | g | 0.199 | 1.465 | |
| Na2SO4 | 2.84 | g | 2.832 | 19.935 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na-3-hydroxybutyrate | 1.40 | g | 1.396 | 11.07 | |
| Yeast extract | 1.00 | g | 0.997 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 4.50 | g | 4.487 | 53.407 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.40 | g | 0.399 | 1.66 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use check pH of complete medium and adjust to 7.2 - 7.4, if necessary. | |||||
Media with this solution
| 314 | ILYOBACTER POLYTROPUS MEDIUM |