Main sol. 316
Internal Solution-ID: S585
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 0.984 | 18.401 | |
| KH2PO4 | 0.3 | g | 0.295 | 2.17 | |
| Na2HPO4 x 12 H2O | 2.8 | g | 2.756 | 19.413 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.197 | 0.968 | |
|
Trypticase peptone
(BD BBL) |
10.0 | g | 9.843 | - | |
|
Yeast extract
(OXOID) |
3.0 | g | 2.953 | - | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
| Wolin's vitamin solution | 5.0 | ml | - | - | |
|
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) |
1.0 | ml | 9.8e-4 | 0.004 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 4.9e-4 | 0.002 | |
| Na2S x 9 H2O | 0.6 | g | 0.591 | 2.459 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% carbon monoxide gas. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. | |||||
Media with this solution
| 316 | MOORELLA THERMOACETICA (TYE-CO) MEDIUM |