Main sol. 317
Internal Solution-ID: S586
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.00 | g | 1 | 18.695 | |
K2HPO4 | 0.40 | g | 0.4 | 2.297 | |
MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
Minerals solution | 50.00 | ml | - | - | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Yeast extract | 1.00 | g | 1 | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 1.00 | g | 1 | 9.435 | |
Methanol | 5.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
Distilled water | 950.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, methanol, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vilas and autoclave. Add methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary. |
317 | METHANOSARCINA THERMOPHILA MEDIUM |