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Trace element solution

Internal Solution-ID: S589

This solution is used in 15 mediaand was first defined in medium 318.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
12.80 g 12.8 66.967
FeCl2 x 4 H2O 1.00 g 1 5.03
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.03 g 0.03 0.231
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiCl2 x 6 H2O 0.10 g 0.1 0.772
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Na2WO4 x 2 H2O 0.04 g 0.04 0.121
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
318a PELOBACTER (FUF) MEDIUM
318c PELOBACTER ACIDIGALLICI MEDIUM
318 METHANOSARCINA THERMOPHILA (BCYT) MEDIUM
318b ANAEROTIGNUM NEOPROPIONICUM MEDIUM
892 METHANOCALCULUS PUMILUS MEDIUM
960 PELOTOMACULUM MEDIUM
963 THERMOANAEROMONAS MEDIUM
1005 THERMODESULFOBIUM MEDIUM
1057 ETHANOLIGENES MEDIUM
1211 CALDISERICUM MEDIUM
1257 METHANOCELLA MEDIUM
1329 METHANOSAETA PELAGICA MEDIUM
1477 CALORAMATOR QUIMBAYENSIS MEDIUM
1487 MAGNETOVIBRIO MEDIUM
1539 THERMOTOGA PROFUNDA MEDIUM