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Modified Wolin's mineral solution II

Internal Solution-ID: S5902

This solution is used in 23 mediaand was first defined in medium 700.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g - -
MgSO4 x 7 H2O 3.000 g - -
MnSO4 x H2O 0.500 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoSO4 x 7 H2O 0.180 g - -
CaCl2 x 2 H2O 0.100 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.010 g - -
AlK(SO4)2 x 12 H2O 0.020 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.010 g - -
NiCl2 x 6 H2O 0.025 g - -
Na2SeO3 x 5 H2O 0.300 mg - -
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
534 MODIFIED SME MEDIUM
700 ALKALIPHILIC SPIROCHAETE MEDIUM
701 HALOANAEROBACTER CHITINOVORANS MEDIUM
761 HALOTHERMOTHRIX ORENII
764 HALOANAEROBIUM LACUSROSEUS MEDIUM
766 THERMOCOCCUS CHITINOPHAGUS MEDIUM
783 HYDROGENOTHERMUS HIRSCHII MEDIUM
806 THERMOCOCCUS MEDIUM
887 OS MEDIUM
889 ISS1 MEDIUM
890 VM1 MEDIUM
891 SME MEDIUM MODIFIED FOR IS7
926 ALKALIPHILIC THERMOCOCCUS MEDIUM
930 NATRONINCOLA HISTIDINOVORANS MEDIUM
932 HALONATRONUM SACCHAROPHILUM MEDIUM
933 HALOANAEROBIUM CONGOLENSE MEDIUM
973 MARINITHERMUS HYDROTHERMALIS MEDIUM
977 VULCANITHERMUS MEDIUM
979 PETROBACTER SUCCINIMANDENS MEDIUM
1024 NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM
1223 TINDALLIA TEXCOCOENSIS MEDIUM
1373 IRD MEDIUM
1573 T 5 MEDIUM