Main sol. 318a
Internal Solution-ID: S590
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HEPES
(SIGMA) |
11.92 | g | 11.79 | 49.477 | |
KH2PO4 | 0.30 | g | 0.297 | 2.181 | |
NaCl | 0.60 | g | 0.593 | 10.155 | |
MgCl2 x 6 H2O | 0.10 | g | 0.099 | 0.487 | |
CaCl2 x 2 H2O | 0.08 | g | 0.079 | 0.538 | |
Trace element solution | 10.00 | ml | - | - | |
NH4Cl | 1.00 | g | 0.989 | 18.492 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
KHCO3 | 2.00 | g | 1.978 | 19.76 | |
2-Furoic acid
(SIGMA) |
2.24 | g | 2.216 | 19.768 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.297 | 1.689 | |
Na2S x 9 H2O | 0.30 | g | 0.297 | 1.235 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except bicarbonate, vitamins, furoic acid, cysteine and sulfide and adjust pH of solution to 7.0. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add furoic acid (stock solution neutralized with NaOH), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary. |
318a | PELOBACTER (FUF) MEDIUM |