MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 318c

Download this solution as

CSV JSON PDF

Internal Solution-ID: S592

Equipment needed: Autoclave Filter Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.297 2.181
NaCl 0.60 g 0.593 10.155
MgCl2 x 6 H2O 0.10 g 0.099 0.487
CaCl2 x 2 H2O 0.08 g 0.079 0.538
Trace element solution 10.00 ml - -
NH4Cl 1.00 g 0.989 18.492
Yeast extract
(OXOID) 		0.50 g 0.495 -
Trypticase peptone
(BD BBL) 		0.50 g 0.495 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
KHCO3 2.50 g 2.473 24.7
Na-gallate 1.90 g 1.879 9.783
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.30 g 0.297 1.689
Na2S x 9 H2O 0.30 g 0.297 1.235
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate, vitamins, gallate, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, gallate, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and gallate are sterilized by filtration. Prior to use adjust pH of complete medium to 7.3, if necessary.
318c PELOBACTER ACIDIGALLICI MEDIUM