Main sol. 935
Internal Solution-ID: S5975
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.997 | 18.639 | |
| K2HPO4 | 0.08 | g | 0.08 | 0.458 | |
| MgCl2 x 6 H2O | 4.50 | g | 4.487 | 22.068 | |
| CaCl2 x 2 H2O | 0.38 | g | 0.379 | 2.577 | |
| NaCl | 30.00 | g | 29.91 | 511.812 | |
| KNO3 | 0.50 | g | 0.499 | 4.931 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 2.00 | g | 1.994 | - | |
| Na2CO3 | 1.50 | g | 1.496 | 14.11 | |
| Na-pyruvate | 0.50 | g | 0.499 | 4.53 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, pyruvate and vitamins), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30-45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate and vitamins from anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The pyruvate and vitamins solutions are sterilized by filtration. The pH of the complete medium should be 6.5 - 6.7. | |||||
Media with this solution
| 935 | DEFERRIBACTER MEDIUM |